phycoerythrin cyanine7 pe cy7 conjugated streptavidin Search Results


pe cy 7 conjugated streptavidin  (Thermo Fisher)
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Thermo Fisher pe cy 7 conjugated streptavidin
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streptavidin apc-cyanine7 (apc-cy7  (Becton Dickinson)
90
Becton Dickinson streptavidin apc-cyanine7 (apc-cy7
Streptavidin Apc Cyanine7 (Apc Cy7, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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streptavidin pe cy7  (SouthernBiotech)
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SouthernBiotech streptavidin pe cy7
Streptavidin Pe Cy7, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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streptavidin pe-cy™7  (Becton Dickinson)
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Becton Dickinson streptavidin pe-cy™7
Streptavidin Pe Cy™7, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2 g/ml allophycocyanin (apc)-cyanine 7 (cy7)-conjugated streptavidin  (Becton Dickinson)
90
Becton Dickinson 2 g/ml allophycocyanin (apc)-cyanine 7 (cy7)-conjugated streptavidin
2 G/Ml Allophycocyanin (Apc) Cyanine 7 (Cy7) Conjugated Streptavidin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phycoerythrin (pe)-cy-7-streptavidin  (Becton Dickinson)
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Becton Dickinson phycoerythrin (pe)-cy-7-streptavidin
Phycoerythrin (Pe) Cy 7 Streptavidin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe-indotricarbocyanine (cy)7-conjugated streptavidin (1:1000)  (Thermo Fisher)
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Thermo Fisher pe-indotricarbocyanine (cy)7-conjugated streptavidin (1:1000)
Pe Indotricarbocyanine (Cy)7 Conjugated Streptavidin (1:1000), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe-cy™7 streptavidin  (Becton Dickinson)
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Becton Dickinson pe-cy™7 streptavidin
Pe Cy™7 Streptavidin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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streptavidin-phycoerythrin (pe)-cyanin (cy)7  (Becton Dickinson)
90
Becton Dickinson streptavidin-phycoerythrin (pe)-cyanin (cy)7
(A) WT and CALM −/− murine embryonic fibroblasts (MEFs) engineered to express KIT were incubated with biotinylated SCF for 60 min, and then with the APC-conjugated <t>streptavidin</t> for 30 min at 4°C. After stripping unincorporated SCF, the amount of the internalized SCF-KIT complex was quantified from the fluorescence intensity at the indicated times. (B) Uptake of SCF in WT or CALM −/− MEFs. The vertical axis indicates the ratio of mean fluorescence intensity, MFI (internalized SCF-KIT complex/initial surface KIT) (Data represent means ± SD, n = 3, n.s.: not significant (p = 0.079)).
Streptavidin Phycoerythrin (Pe) Cyanin (Cy)7, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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streptavidin-conjugated phycoerythrin-cyanine 7  (Becton Dickinson)
90
Becton Dickinson streptavidin-conjugated phycoerythrin-cyanine 7
(A) WT and CALM −/− murine embryonic fibroblasts (MEFs) engineered to express KIT were incubated with biotinylated SCF for 60 min, and then with the APC-conjugated <t>streptavidin</t> for 30 min at 4°C. After stripping unincorporated SCF, the amount of the internalized SCF-KIT complex was quantified from the fluorescence intensity at the indicated times. (B) Uptake of SCF in WT or CALM −/− MEFs. The vertical axis indicates the ratio of mean fluorescence intensity, MFI (internalized SCF-KIT complex/initial surface KIT) (Data represent means ± SD, n = 3, n.s.: not significant (p = 0.079)).
Streptavidin Conjugated Phycoerythrin Cyanine 7, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pe-c7-streptavidin (557598)  (Becton Dickinson)
90
Becton Dickinson pe-c7-streptavidin (557598)
(A) WT and CALM −/− murine embryonic fibroblasts (MEFs) engineered to express KIT were incubated with biotinylated SCF for 60 min, and then with the APC-conjugated <t>streptavidin</t> for 30 min at 4°C. After stripping unincorporated SCF, the amount of the internalized SCF-KIT complex was quantified from the fluorescence intensity at the indicated times. (B) Uptake of SCF in WT or CALM −/− MEFs. The vertical axis indicates the ratio of mean fluorescence intensity, MFI (internalized SCF-KIT complex/initial surface KIT) (Data represent means ± SD, n = 3, n.s.: not significant (p = 0.079)).
Pe C7 Streptavidin (557598), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apc-cy7-coupled streptavidin  (Thermo Fisher)
90
Thermo Fisher apc-cy7-coupled streptavidin
(A) WT and CALM −/− murine embryonic fibroblasts (MEFs) engineered to express KIT were incubated with biotinylated SCF for 60 min, and then with the APC-conjugated <t>streptavidin</t> for 30 min at 4°C. After stripping unincorporated SCF, the amount of the internalized SCF-KIT complex was quantified from the fluorescence intensity at the indicated times. (B) Uptake of SCF in WT or CALM −/− MEFs. The vertical axis indicates the ratio of mean fluorescence intensity, MFI (internalized SCF-KIT complex/initial surface KIT) (Data represent means ± SD, n = 3, n.s.: not significant (p = 0.079)).
Apc Cy7 Coupled Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) WT and CALM −/− murine embryonic fibroblasts (MEFs) engineered to express KIT were incubated with biotinylated SCF for 60 min, and then with the APC-conjugated streptavidin for 30 min at 4°C. After stripping unincorporated SCF, the amount of the internalized SCF-KIT complex was quantified from the fluorescence intensity at the indicated times. (B) Uptake of SCF in WT or CALM −/− MEFs. The vertical axis indicates the ratio of mean fluorescence intensity, MFI (internalized SCF-KIT complex/initial surface KIT) (Data represent means ± SD, n = 3, n.s.: not significant (p = 0.079)).

Journal: PLoS ONE

Article Title: Clathrin Assembly Protein CALM Plays a Critical Role in KIT Signaling by Regulating Its Cellular Transport from Early to Late Endosomes in Hematopoietic Cells

doi: 10.1371/journal.pone.0109441

Figure Lengend Snippet: (A) WT and CALM −/− murine embryonic fibroblasts (MEFs) engineered to express KIT were incubated with biotinylated SCF for 60 min, and then with the APC-conjugated streptavidin for 30 min at 4°C. After stripping unincorporated SCF, the amount of the internalized SCF-KIT complex was quantified from the fluorescence intensity at the indicated times. (B) Uptake of SCF in WT or CALM −/− MEFs. The vertical axis indicates the ratio of mean fluorescence intensity, MFI (internalized SCF-KIT complex/initial surface KIT) (Data represent means ± SD, n = 3, n.s.: not significant (p = 0.079)).

Article Snippet: Then, MNCs were incubated with the antibodies (Abs) as follows: anti-lineage Abs (a cocktail of biotinylated Abs against CD3e (145–2C11), CD45R/B220 (RA3–6B2), Gr-1 (RB6–8C5), and TER-119 (TER-119)), fluorescein isothiocyanate (FITC)-conjugated anti-Sca-1 Ab (D7), allophycocyanin (APC)-conjugated anti-c-KIT Ab (2B8), and streptavidin-phycoerythrin (PE)-cyanin (cy)7 (BD Biosciences).

Techniques: Incubation, Stripping Membranes, Fluorescence

Localization of KIT was analyzed before and after SCF stimulation under confocal microscopy using WT and CALM −/− MEFs engineered to express KIT. KIT was visualized by the biotinylated anti-KIT antibody (Ab) and AlexaFluor 568 streptavidin conjugates.

Journal: PLoS ONE

Article Title: Clathrin Assembly Protein CALM Plays a Critical Role in KIT Signaling by Regulating Its Cellular Transport from Early to Late Endosomes in Hematopoietic Cells

doi: 10.1371/journal.pone.0109441

Figure Lengend Snippet: Localization of KIT was analyzed before and after SCF stimulation under confocal microscopy using WT and CALM −/− MEFs engineered to express KIT. KIT was visualized by the biotinylated anti-KIT antibody (Ab) and AlexaFluor 568 streptavidin conjugates.

Article Snippet: Then, MNCs were incubated with the antibodies (Abs) as follows: anti-lineage Abs (a cocktail of biotinylated Abs against CD3e (145–2C11), CD45R/B220 (RA3–6B2), Gr-1 (RB6–8C5), and TER-119 (TER-119)), fluorescein isothiocyanate (FITC)-conjugated anti-Sca-1 Ab (D7), allophycocyanin (APC)-conjugated anti-c-KIT Ab (2B8), and streptavidin-phycoerythrin (PE)-cyanin (cy)7 (BD Biosciences).

Techniques: Confocal Microscopy